|
KMID : 0386520140230010105
|
|
Research Institute of Korean Medicone
2014 Volume.23 No. 1 p.105 ~ p.114
|
|
|
Comparison on the Antioxidative Activity of Ethanol and Hot Water Extracts of Euphorbia supina Rafinesque
|
|
Kang Ran-Yi
Choi Hak-Joo
Bak Ji-Won
Sim Boo-Yong
Lee Hae-Jin
Kim Dong-Hee
|
|
|
Abstract
|
|
|
Objectives : In this study, the antioxidant activities of the 80% ethanol and hot water extracts of Euphorbia supina Rafinesque were investigated.
Methods : We measured total phenol contents, flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The production of reactive oxygen species was measured in LPS-induced RAW 264.7 cells using flow cytometry system.
Results : The content of phenol in the hot water extract was 65.529 ± 0.462 mg/g and 126.932 ± 1.894 mg/g in the 80% ethanol extract, and that of flavonoid in the hot water extract was 16.063 ± 0.471 mg/g and 29.159 ± 1.963 mg/g in the ethanol extract. The 80% ethanol extract also showed higher DPPH and ABTS radical scavenging activities (90.8±1.0% and 92.5±0.7%) than the hot water extract (81.5±0.5% and 91.5±0.2%). The production of reactive oxygen species(ROS) was reduced dose-dependently by 80% ethanol and hot water extract at concentration of 1, 10 and 100 ㎍/㎖ of RAW 264.7 cells.
Conclusion : According to these results, the 80% ethanol extract of Euphorbia supina Rafinesque has a good anti-oxidative effects than the hot water extract. Thus, the 80% ethanol extract of Euphorbia supina Rafinesque may serve as useful natural antioxidants.
|
|
|
KEYWORD
|
|
|
Antioxidant activity , Euphorbia supina Rafinesque , flavonoid , phenol , radical scavenging , reactive oxygen species
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|